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Amplicon sequencing analysis workflow using DADA2 and QIIME2
| Date | Stars |
|---|---|
| 2026-07-24 | 255 |
| 2026-07-25 | 255 |
| 2026-07-28 | 255 |
| 2026-07-30 | 255 |
| 2026-08-06 | 256 |
Today
+1 stars today
This week
+1 stars this week
This month
— stars this month
Momentum
20.0
growth rate 0.39%/day
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<source media="(prefers-color-scheme: dark)" srcset="docs/images/nf-core-ampliseq_logo_dark.png">
<img alt="nf-core/ampliseq" src="docs/images/nf-core-ampliseq_logo_light.png">
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[](https://github.com/codespaces/new/nf-core/ampliseq)
[](https://github.com/nf-core/ampliseq/actions/workflows/nf-test.yml)
[](https://github.com/nf-core/ampliseq/actions/workflows/linting.yml)[](https://nf-co.re/ampliseq/results)[](https://www.nf-test.com)
[](https://doi.org/10.5281/zenodo.1493841)[](https://doi.org/10.3389/fmicb.2020.550420)
[](https://www.nextflow.io/)
[](https://github.com/nf-core/tools/releases/tag/4.0.2)
[](https://docs.conda.io/en/latest/)
[](https://www.docker.com/)
[](https://sylabs.io/docs/)
[](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/ampliseq)
[](https://nfcore.slack.com/channels/ampliseq)[](https://bsky.app/profile/nf-co.re)[](https://mstdn.science/@nf_core)[](https://www.youtube.com/c/nf-core)[](https://youtu.be/a0VOEeAvETs)
## Introduction
**nfcore/ampliseq** is a bioinformatics analysis pipeline used for amplicon sequencing, supporting denoising of any amplicon and supports a variety of taxonomic databases for taxonomic assignment including 16S, ITS, CO1 and 18S. Phylogenetic placement is also possible. Multiple region analysis such as 5R is implemented. Supported is paired-end Illumina or single-end Illumina, PacBio and IonTorrent data. Default is the analysis of 16S rRNA gene amplicons sequenced paired-end with Illumina.
A video about relevance, usage and output of the pipeline (version 2.1.0; 26th Oct. 2021) can also be found in [YouTube](https://youtu.be/a0VOEeAvETs) and [billibilli](https://www.bilibili.com/video/BV1B44y1e7MM), the slides are deposited at [figshare](https://doi.org/10.6084/m9.figshare.16871008.v1).
<p align="center">
<img src="docs/images/ampliseq_workflow.png" alt="nf-core/ampliseq workflow overview" width="60%">
</p>
On release, automated continuous integraExcerpt of 11,167 characters
Read on GitHubWould you bet a product on this? Bounded 0–100 and slow moving.
matched fp:4afa6353df1ceb54, topic:workflow